Zetalif Laser

01 Overview

Picometrics Zetalif Laser induced fluorescence (LIF) detectors are ultra sensitive solutions for all separation techniques such as Liquid Chromatography (capillary LC, microLC, nanoLC, UPLC) and Capillary Electrophoresis. The modular design of this detector provides maximum versatility and enables an unrivalled level of sensitivity for fluorescence measurement thanks to a broad range of lasers offering an excitation wavelength from 266nm to 785nm. Analysts can adapt the wavelength of the laser depending on their application, simply by changing the laser and the corresponding filter blocks.

02 Technology

Picometrics offers a unique patented collinear optical arrangement making the detector performances unsurpassed.

The key feature of this patented technology is the use of a ball lens in direct contact with a capillary or micro- column, enabling both high fluorescence recovery and ease-of -use. Fluorescence is read directly inside the capillary, without degrading the resolution of the analytical chain. No optical fine tuning is required when changing the capillary.

Moreover, recent upgrade of this unique technology allowed a superb miniaturization of the optical head, linked to the light source and detector by optical fibers. This design makes easy the integration of the detector in any analytical system.

03 About Lasers

Lasers offer the best sensitivity thanks to coherent light and optimal focusing.

A wide range of wavelength is available, with compact and robust solid state lasers, including 266 nm for native fluorescence of proteins.

Lasers must be chosen when sensitivity is critical or when optimal wavelength is not available in the LED range.

In order to provide adequate safety to end users all the lasers have the European Certification (CE) and are certified as complying with the US laser product performance standard and meet the requirements of the US Center for Devices and Radiological Health (CDRH) Office of Compliance.

Lasers can easily be controlled manually or remotely by the detector to provide maximum safety.

04 Wavelength - Application

The Zetalif Laser detector is a sensitive solution for HPLC, fast-LC and CE systems and offers a large range of laser wavelengths:

266 nm: native fluorescence for the detection of proteins
325 nm or 355 nm : mono and polysaccharides labeled with 2AB or 2AA
405 nm or 445 nm: analysis of amino acids labeled with NDA
473 nm or 488 nm: DNA/RNA, immunoglobins, IgG, amino acids, oligonucleotides polysaccharides…
515 nm or 532 nm: immunoglobins, IgG tagged with 5-Tamra-SE
594 nm: analysis of oligonucleotides labeled with Cy3
633 nm or 642 nm: oligonucleotides labeled with Cy5

For other wavelengths, please contact Picometrics.

05 Benefits

  • High Sensitivity: 10 times to 100 times greater than conventional fluorescence and up to 100,000 times more sensitive than UV detection
  • Modularity: Adaptable to CE and HPLC systems including Fast-LC, Capillary LC and nanoLC.
  • Selectivity: Low noise and interference molecules will not be detected, only labeled or intrinsically fluorescent molecules are detected.
  • Versatility:  13 interchangeable different lasers available for any application.

06 Interface with CE, LC, MS

Interface with CE systems

  • with Agilent Technologies 7100 CE
  • with other CE : Sciex P/ACE MDQ and PA 800, PrinCE Next, WynSep Wyn-CE
  • for  other CE, please contact Picometrics

Interface with LC systems

  • compatible with all LC systems (micro, fast-LC)
  • optimal sensitivity is achieved at flow rates < 0.5 mL/min

Interface with MS systems

  • online CE-LIF-MS
  • online LC-LIF-MS
  • detection cell as close as possible to MS system

Contact us for further information….

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